Abstract: Objective To study the safety of low temperature plasma air sterilizer on animals. Methods Peripheral blood of 40 mice was used as the experimental group and randomly divided into two groups. The room of mice in the control group was not disinfected by air disinfecting machine and kept ventilated frequently. The mice in the experimental group were disinfected with low-temperature plasma air sterilizer for 24 hours. The physiological state and physicochemical indexes of the mice in the two groups were observed. Results There was no significant difference in the physiological state of mice in the environment after being disinfected by plasma air disinfectant. There was not different in the indicators of white blood cells, red blood cells, hemoglobin and platelet in the peripheral blood between the two groups (P＞0.05).Conclusion 1. The low temperature plasma air disinfection machine for air purification and disinfection has no effect on the physiological state of mice.2. Low temperature plasma air disinfection machine for air purification and disinfection of white blood cells, red blood cells, hemoglobin, platelet count of mice has no obvious effect.